|
In order to inhibit vitrification of Gypsophila paniculata in vitro, various effects of agar concentrations, growth inhibitors, water potential of media and culture conditions on influencing occurrence and inhibition of vitrification were studied in this experiment. As the concentration of agar was increased, fresh weight, number of shoots and rate of vitrification were decreased. Water potential of media below -6.0 bars (1.3% agar) reduced fresh weight/dry weight ratio significantly. Treatment of growth inhibitors such as CCC, ancymidol and TIBA was not effective in production of glaucous plantlets. Culture condition of 22℃ and 3,000 lux decreased the rate of vitrification significantly, and at 5℃, it was possible to maintain plant in vitro for more than one year without subculture. Different types of closures of culture vessel influenced theoccurrence and growth of vitrified plantlets and good aeration decreased shoot growth but produced glaucous plantlets. Continuous subcultures resulted in increase the rate of vilification and treatment of antitranspirants was not effective in increasing survival rate of vitrified plantlets in vivo.
|